Surface phenotype of splenic DCs from mice treated with FL.

DCs isolated from spleens of C57BL/6 mice that were either untreated or treated for 10 days with muFL or huFL (10 μg/day) were immunofluorescence stained with antibodies directed to typical murine DC markers. The DCs were analyzed by gating on high forward light scatter and high levels of expression of CD11c. The data are representative of more than 5 experiments that all examined pooled splenic DCs from at least 3 mice.