Increase in splenic DCs during ProGP-1 treatment.

CD8⁺ DCs constituted the principal DC population after 2.5 days of treatment. DCs were isolated from spleens of mice that were either untreated or treated with ProGP-1 for 0.5 to 16 days. The DCs were gated on high forward scatter and high expression of CD11c and MHC class II and analyzed for CD4 and CD8 expression; then the number of DCs in each subset was recorded. Data are from 1 experiment but findings at each time point were analyzed on at least 3 occasions, with similar results.