Surface phenotype of splenic DCs from mice treated with ProGP-1.

DCs isolated from spleens of C57BL/6 mice that were either untreated or treated for 10 days with ProGP-1 (20 μg/day) were immunofluorescence stained with antibodies directed to typical murine DC markers and costimulation molecules. The DCs were analyzed by gating on high forward light scatter and high levels of expression of CD11c, MHC class II, or both. Data are representative of more than 10 experiments, all of which examined pooled splenic DCs from at least 3 mice.