Flow cytometric analysis of the binding of FITC-heparin to live, apoptotic, and necrotic cells derived from cultured Jurkat T cells.

Jurkat cells were treated with Camptothecin for 24 hours before labeling with FITC-heparin at a range of concentrations (0.5 nM-5000 nM). Cells were washed and labeled with PE–annexin V and 7-AAD, as described in “Materials and methods.” Live, apoptotic, and necrotic cells were discriminated by FACS analysis, and binding at each concentration of FITC-heparin is shown as relative fluorescence intensity on the x-axis (log scale).