![Fig. 1. 5-HT promotes growth cessation in group 1 BL cells. / (A) L3055 BL cells plated at an initial density of 5 × 104/mL in flat-bottomed microwells, with concentrations of 5-HT ranging from 1 to 150 μM as indicated, cultured for 24 hours, and DNA synthesis (expressed as % control) assessed by [3H]Tdr incorporation over the last 4 hours. (B) As for panel A, but cells plated at different cell numbers indicated per 200 μL with 5-HT at 5 μM (■), 50 μM (○), 150 μM (♦). (C) Four group 1 BL lines (L3055, BL2, Elijah, and Mutu I) and one group 3 cell line (Mutu III) were treated with 125 μM 5-HT, as in panel A. (D) L3055 cells plated at 5 × 104/mL were cultured for 24 hours either alone (black histogram), with 5-HT at 150 μM (open histogram), or with anti-μ chain antibody at 10 μg/mL (shaded histogram), then were harvested for analysis of their cell cycle profile. All data are given as mean ± SEM from 8 separate experiments (A) and from 3 separate experiments (B-D).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/7/10.1182_blood.v99.7.2545/6/h80722355001.jpeg?Expires=1724237080&Signature=ziHfJbL-AxHPqC1zxXvv60YdykJ3sInNK9C0uTWwnKLV2l8Vpwo9-0MFpZCY0Is2jXdArepCLqy6pbcoDy9TfAlefS2N~wM9KzukEOyujunMmtsePNypDp9GkV-oHyOLiLtUv0DackByxY-392NUuDyuRuaR7zSYQLgfiS~H19gxTSE207siSjoYdZjxIDxIqCjrk4fGgnhR4BIqoP~EzvmyRImpE5yQa-9viaDt8N7K-EIPoLpqjSGmpdDI2thlLwR~69nHYxDakRLWQ-2rB~mTdIulS91HG6mw51H3jP-WHJKuEDw9XKjS53AsbcL-CKoDb8vq6Fs5BgUtVur3zQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
5-HT promotes growth cessation in group 1 BL cells.
(A) L3055 BL cells plated at an initial density of 5 × 104/mL in flat-bottomed microwells, with concentrations of 5-HT ranging from 1 to 150 μM as indicated, cultured for 24 hours, and DNA synthesis (expressed as % control) assessed by [3H]Tdr incorporation over the last 4 hours. (B) As for panel A, but cells plated at different cell numbers indicated per 200 μL with 5-HT at 5 μM (■), 50 μM (○), 150 μM (♦). (C) Four group 1 BL lines (L3055, BL2, Elijah, and Mutu I) and one group 3 cell line (Mutu III) were treated with 125 μM 5-HT, as in panel A. (D) L3055 cells plated at 5 × 104/mL were cultured for 24 hours either alone (black histogram), with 5-HT at 150 μM (open histogram), or with anti-μ chain antibody at 10 μg/mL (shaded histogram), then were harvested for analysis of their cell cycle profile. All data are given as mean ± SEM from 8 separate experiments (A) and from 3 separate experiments (B-D).
