Hematopoietic progenitor cell migration was decreased by the transduction of the PH domain or a Gab2 deletion mutant lacking SHP-2 binding sites.
(A) Primary bone marrow cells were transduced with the PH domain and the deletion version of Gab2 cDNAs by retroviral-mediated gene transfer. Transduced hematopoietic cells were sorted for GFP expression by FACS followed by migration assay as described in “Materials and methods.” (B) Primary bone marrow cells from wild-type andviable motheaten mice were directly analyzed for their migration (6 × 105 cells/well) on fibronectin-coated or uncoated transwells, with or without SDF in the lower chambers. Progenitor assays were conducted for the cells before migration and the cells migrating to the lower chambers.
