Fig. 3.
Gab2 is phosphorylated in response to integrin cross-linking.
Ba/F3 cells were stimulated by β1-integrin cross-linking as described in “Materials and methods.” (A) Cell lysates were prepared and anti-Gab2 immunoprecipitation was performed. Immunoprecipitates were resolved by SDS-PAGE and blotted with antiphosphorylated tyrosine, anti-Syk, and anti-Gab2 Abs. (B) Ba/F3 cells were preincubated with 30 and 60 μg/mL piceatannol for 10 minutes or with 3 and 6 μM cytochalasin D for 45 minutes before β1-integrin cross-linking was conducted. Cell lysates were prepared and the phosphorylation status of Gab2 was then examined as above. Shown in both panels are representatives of 2 independent experiments.
