The β1-integrin–mediated adhesion and migration of Ba/F3 cells transfected with dominant negative Gab2 molecules were reduced.
(A) Wild-type, mutant Gab2 cDNAs, the rat IRS-1 PH domain-coding region (IRS) as well as control vector transfected Ba/F3 cells were examined for their capacity to adhere to fibronectin-coated 24-well plates as described in “Materials and methods.” The percentage of adherent cells was determined using the One Solution Proliferation Kit. (B) Migration assays were performed using fibronectin-coated transwells as described in the text. The relative migration (fold change) was expressed as the ratio of the number of wild-type, mutant Gab2 cDNA, and IRS-1 PH domain-transfected cells migrating to the number of GFP vector-transfected cells migrating over the same period of time. Shown in both panels is the mean ± SD of 3 independent experiments; each experiment was performed in triplicate.
