Role of CTLA-4, B7-1, and B7-2 in the suppressor activity of anergic T cells.
CTLA-4 and B7-2 were found to be involved in the suppressor activity of anergic T cells. Alloantigen-specific anergic CD4+ (1 × 105) T cells were cocultured with an equivalent number of activated control T cells (1 × 105) (CTRL) of the same donor and stimulated with mature DCs (2 × 104) from the same DC donor as used during induction of anergy (hatched bars). A coculture of syngeneic control CD4+ T cells (1 × 105) (CTRL) with mature DCs served as controls (black bars). The mAb to CTLA-4, B7-1, and B7-2 or control antibody was added to the coculture experiments as described in “Materials and methods.” T-cell proliferation was measured after 3 days of incubation and an additional 16-hour pulse with 3H-Tdr (37 kBq per well) by means of a liquid scintillation counter. One of 4 independent experiments is shown.
