Fig. 2.
Dose dependence of the suppressor activity.
Suppressor activity of anergic T cells was found to be dose dependent. Different numbers of anergic CD4+ (in the ratios indicated) were restimulated with allogeneic DCs (1 × 104) (generated from the same donor used for the primary culture) in the presence of syngeneic activated CD4+ T cells (1 × 105) stimulated with mature DCs. Proliferation of anergic T cells alone were indicated by an asterisk. Proliferation of T cells was determined by addition of 3H-Tdr after 3 days of culture. Similar results were obtained in 5 independent experiments.
