![Fig. 4. IL-12 potentiates FC/4TOO-induced T-cell responses. / Mice (3 per group) were immunized twice subcutaneously with 1 × 106 4TOO (○), DCs (◊, FC/4TOO (▵), or FC/4TOO combined with intraperitoneal IL-12 (200 ng/mouse) (▴) as depicted in the schema. LNCs (A) and splenocytes (B) were harvested on day 15. The indicated number of cells was incubated with irradiated FC/4TOO cells at a ratio of 10:1 for 5 days. Uptake of [3H]thymidine was measured at 12 hours after a pulse of 0.037 MBq (1 μCi)/well. The stimulation index is presented as the mean ± SD of 3 replicates. T cells from the LNCs (C) and splenocytes (D) were purified through nylon wool and incubated with 4TOO cells at the indicated ratios. CTL activity was determined by the 51Cr release assay.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/7/10.1182_blood.v99.7.2512/6/h80722348004.jpeg?Expires=1724944791&Signature=lWHHELstGJJgYRYTFh2C1loNB9qTX~mUi8MTObTarZOYlX7~ghXE0mtCwcY09bt4gLK-1krQinDuzs3eXg2MIAW0exzxUcYvPxStLkqld4yIU~9BGWHg7zeZVimYIcD1Nf-0UmIZJN4T8gLRV53vquKNg9bD8mbmsJVnELAeEgkR12XbjiNovZSMZBJapXbVtn1~UZdgbSyIEsvBpi3SeO4w5IYTlHqaTzoHigZf8qDm5P-SZMyt65pE35bNAAkztjT3kQ6m9iGjUzMOddvL3j2zXY59XEqMYsd3~NsiJk00BOLNYI~A2ffU6TtJwxOAruYjcxBD6DDtLn4DmwMtHQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-12 potentiates FC/4TOO-induced T-cell responses.
Mice (3 per group) were immunized twice subcutaneously with 1 × 106 4TOO (○), DCs (◊, FC/4TOO (▵), or FC/4TOO combined with intraperitoneal IL-12 (200 ng/mouse) (▴) as depicted in the schema. LNCs (A) and splenocytes (B) were harvested on day 15. The indicated number of cells was incubated with irradiated FC/4TOO cells at a ratio of 10:1 for 5 days. Uptake of [3H]thymidine was measured at 12 hours after a pulse of 0.037 MBq (1 μCi)/well. The stimulation index is presented as the mean ± SD of 3 replicates. T cells from the LNCs (C) and splenocytes (D) were purified through nylon wool and incubated with 4TOO cells at the indicated ratios. CTL activity was determined by the 51Cr release assay.
