Antitumor immunity induced by immunization with FC/4TOO cells.
(A) Balb/c mice (10 per group) were immunized twice subcutaneously with 1 × 106 irradiated 4TOO cells (■), DCs alone (□), FC/4TOO cells (●), or DCs mixed with 4TOO cells (▴). PBS (○) was used as a control. On day 14, the mice were challenged intravenously with 2 × 105 4TOO cells. (B) Balb/c mice (10 per group) were injected intravenously with 2 × 105 4TOO plasmacytoma cells on day 0 and then treated intravenously with irradiated 4TOO cells (□), 1 × 106 FC/4TOO (▴), 2 × 106 FC/4TOO (■), and 5 × 106FC/4TOO (●) on days 2, 6, and 10. (C) Balb/c mice (10 per group) were injected intravenously with 1 × 105 4TOO cells on day 0 and then treated with 2 × 106 FC/4TOO cells (●) on days 2, 6, 10, 35, and 50. Irradiated 4TOO cells were used as a control (□). The mice were monitored for survival. Similar results were obtained in 3 separate experiments.
