Fig. 1.
![Fig. 1. Phenotype and function of FC/4TOO fusion cells. / (A) DCs, 4TOO plasmacytoma, and FC/4TOO fusion cells were analyzed by bidimensional flow cytometry for PKH-26 fluorescence and expression of the indicated antigens. (B) DCs, 4TOO cells, and FC/4TOO fusion cells were analyzed by bidimensional flow cytometry for the expression of MHC class II and Syndecan. (C) DCs (●), 4TOO plasmacytoma (○), FC/4TOO fusion cells (▴), and medium alone (□) were cocultured with C57BL/6 T cells at different ratios for 5 days. [3H]-thymidine uptake was measured at 12 hours after a pulse of 0.037 MBq (1 μCi)/well. The stimulation index is expressed as the mean ± SE of 3 experiments each performed in triplicate.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/7/10.1182_blood.v99.7.2512/6/h80722348001.jpeg?Expires=1724944994&Signature=eLx2O3MldHNeKsOjEXP3aKACv~1QnpSAzwOk8I~2UKjCl5xSjEWsQQIhnI-fF8xKnjCef-jyaHHs3N4Jnst0DR35LhHYVYz2lKmN3yathByZoHCplogIOzsK6BO-pMS0J99z5hyAh6Vf3HLuGJMnd5AdtCP-nPhisOSYAQQtfp7t7qxK2GuBsI9~8B5wzRp5s0dJ5T1Toa6phE993OVsvxbKpbMWPgkwpDTFXswoPsNG1J0uEX0Q2a25vWC8NeiV~8FWxweLoM788mos7zS6nwEfPA2vp-l8Abcy8XOY7oII6WMUo06jwvqD9RmV8WFnqpjC9D72-hODcwibiwUFBQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Phenotype and function of FC/4TOO fusion cells.
(A) DCs, 4TOO plasmacytoma, and FC/4TOO fusion cells were analyzed by bidimensional flow cytometry for PKH-26 fluorescence and expression of the indicated antigens. (B) DCs, 4TOO cells, and FC/4TOO fusion cells were analyzed by bidimensional flow cytometry for the expression of MHC class II and Syndecan. (C) DCs (●), 4TOO plasmacytoma (○), FC/4TOO fusion cells (▴), and medium alone (□) were cocultured with C57BL/6 T cells at different ratios for 5 days. [3H]-thymidine uptake was measured at 12 hours after a pulse of 0.037 MBq (1 μCi)/well. The stimulation index is expressed as the mean ± SE of 3 experiments each performed in triplicate.
