Fig. 2.
In vitro proliferation of parental, mock-transfected, and IL-17–transfected P815 and J558L cells.
Wild-type, mock-transfected (NT-Neo), or IL-17–transfected P815 (A) or J558L (B) cells were plated on 96-well flat-bottomed plates at a density ranging from 102 to 2 × 103 cells per well. The cells were cultured for 3 days, and their proliferation was determined by MTT assay. For these experiments, all cells were cultured in the same medium without G418 or neomycin. For each cell line, a standard curve between the absorbance in the MTT test and the number of cells was determined. Values are mean results from triplicate cultures and are representative of 3 experiments.
