TRF smears from 2 donor/recipient pairs (D/R6 and D/R14).
Three micrograms RsaI and HinfI digests were loaded into a 0.5% agarose gel and electrophoresed overnight alongside 32P-labeled size markers (λHIII; with bands at 23.6, 9.4, 6.6, and 4.4 kb, as indicated). After gel drying, denaturing, and neutralization, gels were hybridized to a γ-32P end-labeled 5′-(CCCTAA)3 telomeric probe at 37°C overnight. After washing, the gel was exposed to a PhosphorImager plate, and each TRF smear was quantified by densitometry using ImageQuant software. Mean TRF lengths for these samples were as follows: D6, 9.2 kb; R6, 8.4 kb, 8.7 kb, and 8.6 kb, at engraftment (1), 6 months (2), and 12 months (3), respectively; D14, 8.6 kb; R14, 8.5 kb, 8.4 kb, 8.4 kb, and 8.4 kb, at engraftment (1), 2 months (2), 6 months (3), and 12 months (4), respectively.
