Fig. 5.
![Fig. 5. Adhesion and migration of cultured CD34+residing in different phases of the cell cycle. / CB CD34+ cells were cultured ex vivo for 48 hours and assayed for Fn binding (n = 18 [A]) or migration toward MS-5 CM across Fn- (n = 6 [B]) or BSA-coated filters (n = 6 [C]). Percent adhesion or migration of cells in G0/G1, S, and G2/M was calculated relative to the total number of cells in each phase in the input population. (A) *Adhesion was statistically higher in S than in G0/G1 (P < .05); **adhesion was statistically higher in G2/M than in G0/G1 and in S (P < .05). (B) *Migration was statistically lower in S than in G0/G1 (P < .05); **migration was statistically lower in G2/M than in G0/G1 and in S (P < .05)](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/6/10.1182_blood.v99.6.2023/6/h80622266005.jpeg?Expires=1724262669&Signature=Xp4we97SeVpAUBiPlSVqq2fon4B6WTfgcnziJPsIAQUNKfgLEkfVw0FJOcbjmdnpBJyXZlbVHKFH4lvSlFqISZZWKftttQXtLbaFzxd8~XE--k0u2OxWqF0oKFEbrRw0drZ2bD5ggH662mE6BxLCJ50575BVnswd~tamoY37N06E-0aN1V8ODq0j~Z6yYjlPn42RE6bzBezEssdocc6vxdjKMTVsKI8dJM7FESyR~BC3VLqJCNeTPcGMJhEY4n9V47gr-zv3QL2GlvDo~x-cXYZqb5~M14zjwVLma1SKUv6x2zqjXrT14S-A4yWcJ1~lwLZwHWlljvNjPzPqNLIgaA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Adhesion and migration of cultured CD34+residing in different phases of the cell cycle.
CB CD34+ cells were cultured ex vivo for 48 hours and assayed for Fn binding (n = 18 [A]) or migration toward MS-5 CM across Fn- (n = 6 [B]) or BSA-coated filters (n = 6 [C]). Percent adhesion or migration of cells in G0/G1, S, and G2/M was calculated relative to the total number of cells in each phase in the input population. (A) *Adhesion was statistically higher in S than in G0/G1 (P < .05); **adhesion was statistically higher in G2/M than in G0/G1 and in S (P < .05). (B) *Migration was statistically lower in S than in G0/G1 (P < .05); **migration was statistically lower in G2/M than in G0/G1 and in S (P < .05)
