Fig. 1.
Transmigration of ex vivo–cultured CD34+cells toward MS-5 CM was enhanced by Fn.
CB CD34+ progenitor cells were stimulated ex vivo during 48 hours and transferred to the top chamber of transwells coated with Fn (10 μg/cm2) or BSA (1%). MS-5 CM or control medium (α-MEM with 10% fetal bovine serum) was placed in the bottom chamber. Cells were allowed to migrate during 3 hours at 37°C (n = 6). *Percent migration was higher across Fn than across BSA (P < .05). **MS-5 CM stimulated migration across both matrices (P < .05).
