Transduction of human PBLs after a short exposure with OKT3SU-displaying lentiviral vectors.

Nonactivated PBLs were incubated for 24 hours with VSV-G–pseudotyped vector particles derived from HIV-1 or from MLV displaying or not displaying OKT3SU chimeric glycoproteins, as indicated. Infected cells were then washed to eliminate unbound viral particles and incubated for 5 days in the presence of human rIL-2 (1 ng/mL) to reduce cell death. The number of GFP⁺ cells was then determined by FACS analysis. Results of transduction in PBLs from 7 different donors are given. The PBL sets correspond to the ones in Figure 5. The MOIs are provided for each experiment.