Fig. 7.
Fig. 7. Influence of HIV-1 accessory genes on the performance of OKT3-displaying lentiviral vectors. / Nonactivated PBLs were incubated with OKT3SU- or OKT3SUx-displaying VSV-G–pseudotyped lentiviral vector generated with either the pCMVΔ8.2 or the pCMVΔ8.91 packaging constructs that express or do not express the HIV-1 accessory genes, respectively. The number of GFP+ cells was determined 6 days after infection by FACS analysis. Results of transduction in PBLs from 3 different donors are given. The PBL sets correspond to those of Figure 5. The MOIs are provided for each experiment.

Influence of HIV-1 accessory genes on the performance of OKT3-displaying lentiviral vectors.

Nonactivated PBLs were incubated with OKT3SU- or OKT3SUx-displaying VSV-G–pseudotyped lentiviral vector generated with either the pCMVΔ8.2 or the pCMVΔ8.91 packaging constructs that express or do not express the HIV-1 accessory genes, respectively. The number of GFP+ cells was determined 6 days after infection by FACS analysis. Results of transduction in PBLs from 3 different donors are given. The PBL sets correspond to those of Figure 5. The MOIs are provided for each experiment.

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