Fig. 5.
Fig. 5. Transduction of human PBLs with OKT3SU-displaying lentiviral vectors. / Resting PBLs were incubated with OKT3SU-displaying lentiviral vector particles (G/OKT3SU) in the absence of activation factors in the cell culture media. As controls, unmodified lentiviral vectors (pseudotyped with VSV-G only) were used to infect the PBLs in the absence of stimuli (G) or in the presence of anti-CD3 (1 μg/mL) soluble antibodies (G + anti-CD3) or in the presence of both anti-CD3 (1 μg/mL) plus anti-CD28 (1 μg/mL) soluble antibodies (G + anti-CD3 + anti-CD28), as indicated. The number of GFP+ cells was determined 6 days after infection by FACS analysis. Results of transduction in PBLs from 7 different donors are given. The MOIs are provided for each experiment.

Transduction of human PBLs with OKT3SU-displaying lentiviral vectors.

Resting PBLs were incubated with OKT3SU-displaying lentiviral vector particles (G/OKT3SU) in the absence of activation factors in the cell culture media. As controls, unmodified lentiviral vectors (pseudotyped with VSV-G only) were used to infect the PBLs in the absence of stimuli (G) or in the presence of anti-CD3 (1 μg/mL) soluble antibodies (G + anti-CD3) or in the presence of both anti-CD3 (1 μg/mL) plus anti-CD28 (1 μg/mL) soluble antibodies (G + anti-CD3 + anti-CD28), as indicated. The number of GFP+ cells was determined 6 days after infection by FACS analysis. Results of transduction in PBLs from 7 different donors are given. The MOIs are provided for each experiment.

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