Immunoblots of pelleted lentiviral vectors generated with VSV-G and OKT3SU- or OKT3SUx-displaying glycoproteins.

Virions were pelleted by ultracentrifugation of supernatants harvested from lentiviral vector-producer cells. Blots were separated at the position of 40-kd marker. The upper portion of the membrane was stained with a mixture of antibodies against MLV-SU and against VSV-G. The lower part of the membrane was stained with antibodies against HIV-1 CA (capsid) protein to assess equivalent loading of virions on the gels. The positions of the OKT3SU, OKT3SUx, VSV-G, and CA proteins are indicated.