Cell size and cell cycle analysis of pre-BCR⁺ and pre-BCR⁻ subpopulations.

(A) Human VpreB⁺ and VpreB⁻CD34⁻subpopulations of CD19⁺κ/λLC⁻ B lineage cells and (B) mouse CD19⁺BP-1⁺κ/λLC⁻ pre-B cells were sorted on the basis of positive or negative cell surface VpreB expression before evaluation of relative cell size by light scatter profile analysis (upper panels) and cell cycle status assessment by Ki-67 expression and DNA content (lower panels).