Analysis of intracellular μHC expression by pre-BCR⁺ and pre-BCR⁻ subpopulations.

(A) Human VpreB⁺ and VpreB⁻CD34⁻subpopulations of CD19⁺κ/λLC⁻ B lineage cells and (B) mouse VpreB⁺ and VpreB⁻subpopulations of B-lineage cells from bone marrow samples were purified by immunofluorescence cell sorting before cell permeabilization and analysis of intracellular μHC expression. Dashed lines indicate intracellular μHC staining; solid lines indicate background fluorescence.