Analysis of SLC expression on human and mouse cell lines.

Pro-B and pre-B cell lines were analyzed for cell surface binding of anti-VpreB and anti-λ5 antibodies by conventional indirect immunofluorescence (dashed line) or by an enhanced immunofluorescence method using fluorochrome-loaded liposomes as a second-step reagent (solid line). Test cells incubated with an excess of unlabeled primary antibodies served as staining controls (shaded histograms). Results illustrated in this and subsequent figures used the VpreB8 anti-human VpreB, R3 anti-mouse Vpre-B, HSL11 anti-human λ5, and LM34 anti-mouse λ5 monoclonal antibodies. Comparable results were obtained with the other anti-VpreB, anti-λ5, and anti-preBCR antibodies used in these studies (see “Materials and methods”).