VEGF protects HL-60 cells from GA-induced apoptosis.

HL-60 cells were cultured in serum-free RPMI, left untreated or treated with VEGF (20 ng/mL), GA (10 μM), or VEGF plus GA every 24 hours, for 72 hours. Apoptotic cells were determined by annexin V staining, as described in “Materials and methods.” Results are shown as a percentage of apoptotic (annexin V⁺) cells. Each determination was done in triplicate. Double asterisks indicate VEGF significantly protects HL-60 cells from GA-induced apoptosis.