VEGF promotes Bcl-2 binding to Hsp90.

Cell extracts were immunoprecipitated with an antibody against Hsp90 and probed with an antibody against Bcl-2. After 12 hours of stimulation, VEGF promoted Bcl-2 binding to Hsp90, an effect that was partially blocked by coincubating the cells with GA (0.02 μM). Western blotting results were quantified by densitometry and are shown as the ratio between densitometry values in treated versus untreated (control) cells. Each experiment was repeated 3 times, and the SD between the different ratios was calculated and is shown. Double asterisks indicate GA significantly blocks Bcl-2 binding to Hsp90 (P < .05).