Fig. 7.
![Fig. 7. Time-dependence of [14C]heme-binding to α1m. / Approximately 0.2 nmol plasma-α1m, t-α1m, human serum albumin (HSA), and orosomucoid was incubated with 25 pmol [14C]heme for 1, 3, 15, 60, and 180 minutes. The binding of [14C]heme to the proteins was then determined by SDS-PAGE, staining (A), and phosphoimaging (B).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/6/10.1182_blood.v99.6.1894/6/h80622232007.jpeg?Expires=1722565582&Signature=GUaumQ7IvhmsWqS45W1p1-cOMsgzRTLKxIJjuQMFDUi0dbsBq711xheIwcqacxzOpq2Z3d2FRSOPw~ZDiiE8V-1hQiTSrYekERkppbpxg9hEr3AzAbULYgIWGRILTSxV2n0d4mvthzCM82u64pHECpcrmwZWfU5WPZt2Oxpgt5Wqz810ZWgNrrkZQq5dL53hBSTY8SD9t7L99hwaIsnPGKcUr7i5sYhw0hfSQjexdV86PM7ff~JNuscL-5ZKhCYNKMb9yl6FAXDWiL1BpY5FZyv4C8xgS-BV5jc~OD5RToab8WgiLTIOk7e8ItPYoNUHSMkYjmThL~VlSSeMabJQgQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Time-dependence of [14C]heme-binding to α1m.
Approximately 0.2 nmol plasma-α1m, t-α1m, human serum albumin (HSA), and orosomucoid was incubated with 25 pmol [14C]heme for 1, 3, 15, 60, and 180 minutes. The binding of [14C]heme to the proteins was then determined by SDS-PAGE, staining (A), and phosphoimaging (B).
