Fig. 2.
Release of the C-terminal tetrapeptide from α1m after cleavage with erythrocyte membranes and oxyhemoglobin.
Plasma-α1m (lanes 1 and 3) was incubated with erythrocyte membranes (lane 2) or oxyhemoglobin (lane 4) as described in “Materials and methods.” After incubation for 3 hours at 37°C, α1m was purified from the reaction mixtures by affinity chromatography and gel chromatography on Sephacryl S-300. Purified α1m, 1 μg to 3 μg, was separated by SDS-PAGE in the presence of mercaptoethanol, staining, and Western blotting with antibodies against α1m or LIPR.
