Fig. 1.
Cleavage of α1m and control proteins by erythrocyte membranes.
Plasma-α1m, IgA-α1m, and orosomucoid were incubated with purified erythrocyte membranes for 2 hours at 37°C. A quantity of 1 μg to 3 μg of each protein was separated by SDS-PAGE (T = 12%, C = 3.3%) without prior affinity chromatography purification of the α1m-components and stained with Coomassie Brilliant Blue. Lane 1 shows the proteins alone and lane 2 shows the proteins with added erythrocyte membranes. The electrophoresis was performed in the presence of mercaptoethanol. Molecular masses of standards are given in kilodaltons.
