GATA-2 expression is significantly decreased in the VAD embryo.

Normal (A-C) and VAD embryos (D-F) were analyzed by whole-mount in situ hybridization for GATA-2 transcripts at stages 7 (A, D), 9 (B, E), and 11 (C, F). All embryos are arranged anterior to the top, with a ventral view. HF indicates head-fold; H, head. Note that GATA-2 transcripts are detected readily in the caudal region of the embryo and blood island region of normal embryos by stage 7, whereas these levels are significantly decreased in the VAD embryos. Original magnification × 32.