Fig. 2.
![Fig. 2. Quantification of hemoglobin content and morphology of erythroid cells in VAD and normal embryos. / Erythroid cells were isolated from normal or VAD embryos at approximately stage 15 (55-hour incubation), and the total number of benzidine-staining cells was counted using a hemacytometer (inset, n = 3 embryos each). Alternatively, total hemoglobin was extracted and measured as shown in the graph (n = 21 [normal] or 33 [VAD]). As indicated by the representative cell in the inset (arrow), there is a high incidence from VAD embryos of cells with abnormal membrane morphology (17.6% compared to 2.2% for normal embryos; n = 1000 cells). Original magnification × 1000.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/7/10.1182_blood.v99.7.2379/6/h80722343002.jpeg?Expires=1724254404&Signature=ptZWGQL2e29viyKH2VITbiqbCGDl8N1IlOSIIBjHPcq2PrR3pYT~LPAkMBJU6bAuhdVgod5KPyWWj8mXOHs-RVRYfrTCvA-mPSGyFvNP-s2WxSH~ukiEx~Loe~7HcHS9NgXheYJoc-AiqA6McQN9BZzKIbzHYvbZHtYP6RRCCg3itDNUdg6gdgK6h~uovuQoc-2Cf0AyDtMeuzkYpjaUt-Hm58ygk1WyT6DFA~f92pSdr7NbZQDb8K08jCSVR9cgDlXMfrMfPuleUC-YVrvPzVprtAv7oHd1-lEym25uAR7Zbbx3l4A6mEYtGTliwF3Gr0sgUzhAiwLWjPJViZ-X5Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Quantification of hemoglobin content and morphology of erythroid cells in VAD and normal embryos.
Erythroid cells were isolated from normal or VAD embryos at approximately stage 15 (55-hour incubation), and the total number of benzidine-staining cells was counted using a hemacytometer (inset, n = 3 embryos each). Alternatively, total hemoglobin was extracted and measured as shown in the graph (n = 21 [normal] or 33 [VAD]). As indicated by the representative cell in the inset (arrow), there is a high incidence from VAD embryos of cells with abnormal membrane morphology (17.6% compared to 2.2% for normal embryos; n = 1000 cells). Original magnification × 1000.
