Fig. 3.
Fig. 3. Messenger RNA for PRDI-BF1/Blimp-1 and BSAP expression levels in different B-cell fractions. / Relative amounts of PRDI-BF1/Blimp-1 and BSAP mRNAs were determined by RT-PCR from highly purified peripheral blood B lymphocytes (PB-B), follicular mantle B cells (FM-B), germinal center B cells (GC-B), tonsil plasma cells (T-PC), peripheral blood plasma cells (PB-PC), and bone marrow plasma cells (BM-PC). RT-PCR using β-actin mRNA sequences served as an internal standard. As molecular weight marker aHinfI digestion of pBR322 was used (MW). Data are representative of the results of 3 experiments using different donors.

Messenger RNA for PRDI-BF1/Blimp-1 and BSAP expression levels in different B-cell fractions.

Relative amounts of PRDI-BF1/Blimp-1 and BSAP mRNAs were determined by RT-PCR from highly purified peripheral blood B lymphocytes (PB-B), follicular mantle B cells (FM-B), germinal center B cells (GC-B), tonsil plasma cells (T-PC), peripheral blood plasma cells (PB-PC), and bone marrow plasma cells (BM-PC). RT-PCR using β-actin mRNA sequences served as an internal standard. As molecular weight marker aHinfI digestion of pBR322 was used (MW). Data are representative of the results of 3 experiments using different donors.

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