American Society of Hematology

Fig. 8.

$Fig. 8. Determination of the amount of heparin bound by Protein A Sepharose–bound antibodies. / Periplasmic fractions of antiheparin antibodies were allowed to bind to Protein A Sepharose beads. The beads were washed and the antibody-bead complexes were incubated with FLUOS-labeled heparin. After washing, the fluorescence of the bound heparin was determined.$

Determination of the amount of heparin bound by Protein A Sepharose–bound antibodies.

Periplasmic fractions of antiheparin antibodies were allowed to bind to Protein A Sepharose beads. The beads were washed and the antibody-bead complexes were incubated with FLUOS-labeled heparin. After washing, the fluorescence of the bound heparin was determined.

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