American Society of Hematology

Fig. 5.

$Fig. 5. Microscopical analysis of renal cortex and pancreas stained with antiheparin antibodies. / Cryosections of rat renal cortex (A-C) and pancreas (D) were incubated with periplasmic fractions containing antiheparin antibody EW4D5 (A, B) or antiheparin antibody EW4E9 (C, D). Bound antibodies were visualized by incubation with anti-VSV mouse monoclonal antibody P5D4, followed by Alexa 488–conjugated goat antimouse IgG. Small arrow in A indicates smooth muscle cells; large arrow in A, endothelium; arrow in B, peritubular capillary; asterisk in B, glomerulus; arrow in C, peritubular capillary. Note the absence of staining of endothelium in A, but staining of endothelium in B. Scale bar, 50 μm.$

Microscopical analysis of renal cortex and pancreas stained with antiheparin antibodies.

Cryosections of rat renal cortex (A-C) and pancreas (D) were incubated with periplasmic fractions containing antiheparin antibody EW4D5 (A, B) or antiheparin antibody EW4E9 (C, D). Bound antibodies were visualized by incubation with anti-VSV mouse monoclonal antibody P5D4, followed by Alexa 488–conjugated goat antimouse IgG. Small arrow in A indicates smooth muscle cells; large arrow in A, endothelium; arrow in B, peritubular capillary; asterisk in B, glomerulus; arrow in C, peritubular capillary. Note the absence of staining of endothelium in A, but staining of endothelium in B. Scale bar, 50 μm.

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