American Society of Hematology

Fig. 4.

$Fig. 4. Confocal microscopical analysis of renal cortex and testis stained with antiheparin antibodies and its sensitivity to heparinase III digestion. / Nontreated (A, C) and heparinase III–treated (B, D) cryosections of rat renal cortex, and nontreated (E, G) and heparinase III–treated (F, H) cryosections of rat testis were incubated with periplasmic fractions containing antiheparin antibody EW4B5 (A, B), antiheparin antibody EW4E1 (C-F) or antiheparin antibody EW4G2 (G, H). Bound antibodies were visualized by incubation with anti-VSV mouse monoclonal antibody P5D4, followed by Alexa 488–conjugated goat antimouse IgG. Note the difference in staining of the glomerulus in A and C and the difference in staining intensity after heparinase III digestion in B, D, F, and H. Scale bar, 100 μm.$

Confocal microscopical analysis of renal cortex and testis stained with antiheparin antibodies and its sensitivity to heparinase III digestion.

Nontreated (A, C) and heparinase III–treated (B, D) cryosections of rat renal cortex, and nontreated (E, G) and heparinase III–treated (F, H) cryosections of rat testis were incubated with periplasmic fractions containing antiheparin antibody EW4B5 (A, B), antiheparin antibody EW4E1 (C-F) or antiheparin antibody EW4G2 (G, H). Bound antibodies were visualized by incubation with anti-VSV mouse monoclonal antibody P5D4, followed by Alexa 488–conjugated goat antimouse IgG. Note the difference in staining of the glomerulus in A and C and the difference in staining intensity after heparinase III digestion in B, D, F, and H. Scale bar, 100 μm.

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