Ca⁺⁺-dependent association of synexin and sorcin with nanovesicular lipid rafts.

Nanovesicles prepared according to method B (ultracentrifugation) were lysed with cold 1% Triton X-100 in TBS, in the presence of Ca⁺⁺ or EDTA, and subjected to discontinuous density gradient centrifugation. Thirteen fractions were collected from the top, and aliquots were analyzed by 12% polyacrylamide gel electrophoresis/silver staining (panel A) and immunoblotting (panel B), as indicated, and for AChE activity and cholesterol content (percentage of total).