American Society of Hematology

Fig. 1.

$Fig. 1. Oligoclonal expansion of TCRβ after stimulation with β2GPI. / PCR-amplified TCRβ products of unstimulated, GP-F–stimulated, and MalBP-stimulated T cells were fractionated on 5% polyacrylamide–glycerol gels and stained with silver. Oligoclonally expanded single-strand DNA bands present in GP-F–stimulated T cells, but not in unstimulated or MalBP-stimulated T cells, were identified as DNAs encoding TCRβ chains of β2GPI-reactive T cells.$

Oligoclonal expansion of TCRβ after stimulation with β₂GPI.

PCR-amplified TCRβ products of unstimulated, GP-F–stimulated, and MalBP-stimulated T cells were fractionated on 5% polyacrylamide–glycerol gels and stained with silver. Oligoclonally expanded single-strand DNA bands present in GP-F–stimulated T cells, but not in unstimulated or MalBP-stimulated T cells, were identified as DNAs encoding TCRβ chains of β₂GPI-reactive T cells.

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