IFN-γ release by in vitro–induced anti-ALK CTL lines.

Autologous EBV-transformed B cells (LCL) (A) and T2 cells (B) were pulsed with 5 μM, respectively, p280-89 and p376-85 peptides as well as influenza matrix peptide (FLU: irrelevant peptide) and were then used as stimulator in an IFN-γ release assay. Preincubation with 5 μg/mL anti-HLA-A2 mAb CR11.351 inhibits IFN-γ secretion. Results of 1 representative experiment of at least 3 performed are shown.