The spleen demonstrated reversal of pathophysiology in engrafted animals.

(A) Spleen weight expressed as percent total body weight (mean ± SEM) in C57BL/6 control (░, n = 5), untreated sickle (▪, n = 7), and engrafted (■, n = 4) animals. Spleen weights in engrafted animals were measured 5 months after transplantation. Spleen weights for control mice were age-matched to the engrafted animals. (B) The balance of hematopoiesis in the spleen was also normalized in engrafted mice. The percent RBCs, reticulocytes, and WBCs (mean ± SEM) all approach C57BL/6 control values (░, n = 5) in engrafted mice (■, n = 4), while untreated sickle animals (▪, n = 7) are highly abnormal. Splenic hematopoiesis in engrafted animals was measured 5 months after transplantation. Control mice were age-matched to the engrafted animals.