LMP-2/HLA-A2 tetramer⁺ CTLs coexpress the 14.G2a-ζ chimeric receptor.

On day 14 after transduction, CTLs were stained with monoclonal antibody 1A7 and FITC-labeled goat antimouse antibody, then incubated with PE-labeled LMP-2/HLA-A2 tetramer, followed by staining with PerCP-labeled anti-CD8 antibody. One million events were acquired and analyzed. Indicated are the absolute numbers of events for tetramer-positive cells that express detectable levels of 14.G2a-ζ in a population of nontransduced CTLs (A) or 14.G2a-ζ–transduced CTLs (B).