Transduced CTLs express surface 14.G2a-ζ chimeric receptors.

Cells of a representative EBV-specific CTL line, 5 days after retroviral transduction with 14.G2a-ζ chimeric receptor genes, were stained with 14.G2a idiotype-specific monoclonal antibody 1A7, followed by incubation with FITC-labeled goat antimouse antibody, and then PerCP-labeled anti-CD or anti-CD4 antibody. Surface immunofluorescence was analyzed by flow cytometry. Panels A and C are nontransduced CTLs; panels B and D are transduced CTLs.