IL-10 mRNA and protein expression in C/EBPε−/− macrophages.

(A) RT-PCR was performed on cDNA from peritoneal lavages of wild-type and C/EBPε−/− mice. (B) Total cell lysates were obtained from bone marrow and peritoneal lavage 72 hours after thioglycollate injection. Western blotting was performed using polyclonal anti–IL-10 antibody and anti–GAPDH antibody (control).