Expression of macrophage-related genes.

Total RNA was harvested from wild-type and C/EBPε-deficient mice peritoneal lavage (PL) and bone marrow–derived macrophages with and without stimulation with LPS + IFN-γ. Northern blot analysis was performed on 10 μg total RNA. Hybridization with GAPDH confirmed equivalent loading of RNA. Fold change was measured by densitometry and was normalized with the GAPDH ratio.