Fig. 13.
![Fig. 13. EBNA 3a-specific T cells determined by tetramer analysis and by IFN-γ production of an HLA-B*0801+ donor. / (A) Cells were triple stained with anti-CD3, anti-CD8, and HLA tetramer (EBNA 3a [QAKWRLQTL]). (B) Cells were additionally stained with anti-CD62L. (C) Cells were stained with anti-CD3, anti-CD8, and anti–IFN-γ after secondary sensitization with EBNA 3a (QAKWRLQTL) peptide-pulsed autologous PBMCs. (D) Cells were additionally stained with anti-CD69. (E, F) Cells were stained after stimulation with autologous EBV BLCL. Each plot is gated on live CD3+CD8+ T cells stained with the respective tetramer or anti–IFN-γ antibody. Percentage of specific CD8+ cells is indicated in the upper quadrant of each plot.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/5/10.1182_blood.v99.5.1730/5/h80522213013.jpeg?Expires=1722413725&Signature=d0JrqL9mOfu3szKqG9L2Skvm5ghecTHGHucvMqOXRDpRCLY4u~atIEQRCOkL5u3Qwo3qQFil-Zc~U00zM7dwts6-~5YSioqWe0umXNGCOEaXmqpItkJHQ5NsciSn1Jis2PAikQsmwkoy1zgr0UUkP9CiKT-9jFEXZhiqbU3EDaqsS2olKDPfjoqxw9csKTZELKghBodsUYHmwlM-p8TKRyB6ovMd0PO3hnzecSwypbJwGB3eIpVNfqKQ4jfg6CY6V1S9MHZQJt5u4y0lYLm9~gGY6DJoQLcILXs4~TzPRk8xG1NzMHinAUTGo~A7Rgx49otQ6SzjrBk4rYfxc-A7Dw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
EBNA 3a-specific T cells determined by tetramer analysis and by IFN-γ production of an HLA-B*0801+ donor.
(A) Cells were triple stained with anti-CD3, anti-CD8, and HLA tetramer (EBNA 3a [QAKWRLQTL]). (B) Cells were additionally stained with anti-CD62L. (C) Cells were stained with anti-CD3, anti-CD8, and anti–IFN-γ after secondary sensitization with EBNA 3a (QAKWRLQTL) peptide-pulsed autologous PBMCs. (D) Cells were additionally stained with anti-CD69. (E, F) Cells were stained after stimulation with autologous EBV BLCL. Each plot is gated on live CD3+CD8+ T cells stained with the respective tetramer or anti–IFN-γ antibody. Percentage of specific CD8+ cells is indicated in the upper quadrant of each plot.
