Fas and Fas ligand expression.

(A) Fas expression in 4 normal cultures (■) and 8 MDS (▪) cultures analyzed by flow cytometry. (B) Modulation of Fas expression by incubation with 1000 IU/mL IFN-γ or 10 ng/mL TNF-α for 16 hours in 4 normal and 8 MDS samples. Results are the ratios of fluorescence intensities (RFI) for specific and irrelevant isotype-matched antibody. The asterisk indicates P < .05. (C) Fas ligand analyzed by Western blotting. Antitubulin antibody was used to check protein loading. Results are representative of analyses of 3 normal and 5 MDS samples.