Fig. 5.
Fig. 5. Epo signaling in MDS and normal erythroid progenitors. / Cells were removed from serum and cytokines for 4 hours at 37°C and then stimulated for 10 minutes with 10 IU/mL Epo at 37°C. (A) Kinetics of Epo-induced STAT5 activation analyzed by electrophoretic mobility shift assay. (B) Western blot analysis of phospho-Akt, phospho-ERK 1/2, and phospho-FKHRL1 on day 12. Western blot results with Akt, ERK 1/2, and FKHRL1 were used as controls for protein loading. Data are representative results from 3 normal and 5 MDS samples.

Epo signaling in MDS and normal erythroid progenitors.

Cells were removed from serum and cytokines for 4 hours at 37°C and then stimulated for 10 minutes with 10 IU/mL Epo at 37°C. (A) Kinetics of Epo-induced STAT5 activation analyzed by electrophoretic mobility shift assay. (B) Western blot analysis of phospho-Akt, phospho-ERK 1/2, and phospho-FKHRL1 on day 12. Western blot results with Akt, ERK 1/2, and FKHRL1 were used as controls for protein loading. Data are representative results from 3 normal and 5 MDS samples.

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