Fig. 3.
Specific CD28 blockade reduces proliferation in secondary MLR.
Human PBMCs (107) were stimulated with 107allogenic irradiated PBMCs. After 7 days, cells were washed and cultivated for 3 days. Then 2 × 104 primed cells were restimulated with 2 × 104 donor-irradiated PBMCs with 10 μg/mL control IgG, anti–human CD28 Fab fragments, or 5 μg/mL CTLA4 immunoglobulin. Proliferation was measured after 3 days. Mean ± SD of triplicate results from 1 of 4 representative experiments is shown. *indicates significative at P < .05.
