Fig. 2.
Specific CD28 blockade reduces cytokine production in primary MLR.
Human PBMCs (105) were stimulated with 105allogenic irradiated PBMCs with 10 μg/mL control IgG, anti–human CD28 Fab fragments, or 10 μg/mL CTLA4 immunoglobulin. Total mRNA was extracted from 48 wells after 5 days, retrotranscribed, and analyzed by quantitative polymerase chain reaction. Results (mean ± SD of 3 measurements) are relative numbers of transcripts reported to HPRT.
