Effect of AT on increase in the lung level of iNOS mRNA 180 minutes after ET administration in rats.

AT (250 U/kg) was administered intravenously 30 minutes prior to ET (5 mg/kg) administration. Control animals were administered saline alone. The iNOS mRNA expression in the lungs was determined 180 minutes after ET administration. (A) Chemiluminograms of typical expression of iNOS mRNA (4.5 kb) in each experimental group; (B) ethidium bromide staining of 18S ribosomal RNA (1 μg of total RNA per each lane). (C) The chemiluminograms of expression of iNOS mRNA were quantified by comparison with the mean value seen in the ET plus saline group, arbitrarily set at 100. Data are expressed as means ± SD of 3 animals. *P < .01 versus control. †P < .05 versus ET plus saline.