IFN-γ production from freshly isolated splenocytes.

(A) Splenocytes were obtained from either wild-type or tyk2-deficient mice and plated in wells with medium alone, IL-12, or IL-18. Supernatants were collected after 48 hours, and the amount of IFN-γ was quantified by ELISA. (B) Splenocytes were stimulated with IL-12 and IL-18. Supernatants were collected after 48 hours, and the amount of IFN-γ was quantified by ELISA.